Immobilization of Glycine Max Amylase onto Variety of Chlorinated and Nitrated Fabrics (Silk, Nylon and Cotton)

Abstract

Amylase is commonly known as starch
hydrolyzing enzyme which degrades the starch into
glucose monomers and limit dextrins by digesting it
from the non-reducing end. Hence, this property of
amylase makes it most industrial important enzyme
with wide applications in food, pharmaceutical, paper,
leather, detergent and textile industries. As well as,
enzyme immobilization is excellent way to bind the
enzyme of interest onto stable carrier systems which
was makes the immobilized enzyme more resistant
towards pathogenic attack on storage as compared to
free enzymes. Hence, immobilization increases the
storage stability and reusability of bound enzyme for
various continuous chemical and biological processes.
In the present work, immobilization of Glycine max
amylase was done onto variety of activated fabrics (silk,
cotton and nylon) whose activation was made by
treating them with sodium chloride and sodium nitrate.
Kinetic parameters (pH, incubation time, temperature,
substrate concentration and CaCl2 concentration) were
studied for Glycine max amylase enzyme by
conventional dinitrosalicylic acid method. This amylase
was found to be thermal stable and showed stability
upon wide range of pH. A comparative study was done
to know % of retention of activity of Glycine max
amylases on to the variety of chosen activated fabrics.