Visualizing the distribution of proteins and estimating their kinetic parameters using Virtual Photoactivated Fluorescence (VPAF)

  • Gustavo Carrero
  • Carlos Contreras
  • Michael J. Hendzel

Abstract

Photoactivated Fluorescence (PAF) and Fluorescence Recovery After Photobleaching (FRAP) are two inverse related fluorescence microscopy techniques used to
study the kinetic of nuclear proteins. In this paper, we will propose and use a method, Virtual Photoactivated Fluorescence (VPAF), based on the inverse relationship between PAF and FRAP, to visualize and quantify the dynamics of proteins within the cell nucleus. In particular, we will visualize the heterogeneous distribution of splicing factors throughout the cell nucleus after virtual photoactivation and estimate kinetic parameters of linker histones using VPAF data.

Published
2018-05-09
How to Cite
CARRERO, Gustavo; CONTRERAS, Carlos; J. HENDZEL, Michael. Visualizing the distribution of proteins and estimating their kinetic parameters using Virtual Photoactivated Fluorescence (VPAF). GSTF Journal of BioSciences (JBio), [S.l.], v. 1, n. 2, may 2018. ISSN 2251-3159. Available at: <http://dl6.globalstf.org/index.php/jbio/article/view/1461>. Date accessed: 17 dec. 2018.