Visualizing the distribution of proteins and estimating their kinetic parameters using Virtual Photoactivated Fluorescence (VPAF)
Photoactivated Fluorescence (PAF) and Fluorescence Recovery After Photobleaching (FRAP) are two inverse related fluorescence microscopy techniques used to
study the kinetic of nuclear proteins. In this paper, we will propose and use a method, Virtual Photoactivated Fluorescence (VPAF), based on the inverse relationship between PAF and FRAP, to visualize and quantify the dynamics of proteins within the cell nucleus. In particular, we will visualize the heterogeneous distribution of splicing factors throughout the cell nucleus after virtual photoactivation and estimate kinetic parameters of linker histones using VPAF data.