In vitro regeneration of Dioscorea hispida through nodal explants – A rich source of starch

Abstract

Dioscorea hispida subterranean tubers that can produce industrial starch and edible flour [3].lForest dwellers collect and process tubers to prepare baichandi chips, which is now a days scarce and a costly commodity. Therefore, to meet the demand, micropropagation technique was applied for the mass multiplication of this species. In the present study regeneration studies has been conducted and attempt has been made to evaluate the effect of adjuvants on in vitro shoot proliferation. The nodal segments of Dioscorea hispida was established in WPM medium supplemented with 1.0 mg/l BAP. Various additives were added in the medium to increase shoot multiplication in D. hispida. The highest number of shoot proliferation was obtained when nodes of Dioscorea hispida were placed on WPM supplemented with 1.0 mg/l BAP and 1000.0 mg/l PVP. Maximum root induction were obtained when the in vitro grown shoots of D. hispida placed on half - WPM with 2.0 mg/l IAA.Rooted plantlets were acclimatized with 90 % survival rate.