Bisphenol A Depresses the Duodenal and Cardiac Movement through Nitric Oxide Mediated Guanylyl cyclase Signal Pathway

Abstract

Humans are exposed to Bisphenol A (BPA) contaminated packaged drinking water, foods and beverages. The effects of BPA on the function of intestinal smooth muscle and cardiac myocytes have been examined in this study. The record of the movements of isolated intestine and perfused heart of toad in response to single dose acute exposure of BPA have been recorded with isotonic transducer coupled to the RMS Polyrite-D. We found significant depression of the movement of duodenum and heart in a dose dependent manner. Further, it was observed that sodium nitroprusside (SNP), a nitric oxide (NO) donor, significantly potentiated the BPA induced inhibition of duodenal and cardiac movement. However, methylene blue (MB), a guanylyl cycalse (GC) inhibitor, and Nω-nitro-L-arginine methyl ester hydrochloride (L-NAME), a nitric oxide synthase (NOS) inhibitor, significantly counteracted the BPA induced inhibition of duodenal and cardiac movement. Thus, we may conclude that BPA depresses the duodenal and cardiac movement through NO-mediated GC signal pathway.